: Select the specific run or order. Look for a link labeled "Download All Results" or "Download .zip" . This package usually includes the .ab1 trace files, .seq text files (FASTA format), and sometimes a .pdf report. 2. Opening and Viewing the Data
: FinchTV (Windows/Mac) and Chromas Lite (Windows) are popular for basic viewing and manual base-call editing.
: Go to your Order History or Data Downloads section. Download AB1 zip
You cannot view the raw chromatogram in a standard text editor. You need specialized software to interpret the visual peaks:
: Always store the original .ab1 files. While .seq files are easier to copy/paste, the .ab1 file is essential for verifying any ambiguous base calls. : Select the specific run or order
An (also known as a trace file) is the standard output format for Sanger DNA sequencing, containing the raw data, base calls, and the chromatogram (the visual "peaks" of the sequence). Scientists typically download these in a compressed ZIP archive when receiving batch results from a sequencing facility. 1. How to Download Your AB1 ZIP
: Look for high, distinct peaks. Overlapping peaks (noise) or "flat" lines usually indicate a failed sequencing reaction or poor template quality. You cannot view the raw chromatogram in a
: If you are a bioinformatician, tools like the sangeranalyseR package in R can process an entire directory of AB1 files from your unzipped archive at once.