: Cells must be in a single-cell suspension to avoid clogging the thin fluidic channels used for imaging.
: It allows for the quantification of intracellular features, such as the translocation of signals into the nucleus or the internalization of particles. Key Protocol Steps Imaging Flow Cytometry: Methods and Protocols
: If internal markers (e.g., nuclear proteins) are the target, cells are fixed with formaldehyde and permeabilized with detergents like Triton X-100. : Cells must be in a single-cell suspension